RNGTT (RNA guanylyltransferase and 5'-phosphatase), also known as HCE, HCE1, hCAP or CAP1A, is a 597 amino acid protein that localizes to the nucleus and exists as four alternatively spliced isoforms.
Expressed in kidney, lung, heart, brain, liver, testis, skin and muscle, RNGTT acts as a bifunctional mRNA-capping enzyme that exhibits RNA 5'-triphosphatase activity at its N-terminus and mRNA guanylyltransferase activity at its C-terminus.
Via its catalytic activity, RNGTT facilitates the first two steps of mRNA cap formation, naming the removal of a y-phosphate from the end of nascent mRNA and the subsequent transfer of the phospho residue to the 5’-diphosphate terminus.
The gene encoding RNGTT maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RNGTT. Species Crossreactivity: Human, mouse, rat
