RNase1 (ribonuclease, RNase A family, 1 (pancreatic), Rib1) gene encodes a 156 amino acid member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily.
RNase 1 endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases.
RNase 1 prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0.
RNase 1 is monomeric and more commonly acts to degrade ds-RNA over ss-RNA.
RNase1 is a digestive enzyme that has been recognized to be one of the most attractive model systems for molecular evolutionary studies.
The RNase1 gene is conserved in chimpanzee, dog, cow, mouse and rat, and maps to human chromosome 14q11.2, where it is linked to seven other RNase A superfamily genes.
The entire RNase A cluster spans 368 kb.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RNASE1. Species Crossreactivity: Human
