The death domain is a cytoplasmic domain of ~80 amino acids that is necessary for the transduction of apoptotic signals and is present in the apoptosis-mediating receptors TNF-R1 and FAS.
Other death domain-containing, but otherwise structurally unrelated proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS.
One of these proteins, the receptor-interacting protein 3 (RIP3), contains an N-terminal kinase domain and shares extensive homology with RIP and RIP2.
However, RIP3 contains a unique C-terminal death domain, which promotes apoptosis.
RIP3 can be expressed as two splice variants, RIP3beta and RIP3γ, which contain a truncated N-terminal kinase domain and a distinct and shorter C-terminus.
Subsequently, expression of these splice variants downregulates RIP3-mediated apoptosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RIP3. Species Crossreactivity: Mouse
