84-2390-65　［取扱停止］RIN1 (Ras and Rab Interactor 1, Ras Inhibitor JC99, Ras interaction/interference Protein 1) 50ul　225559

特徴

• Ras is a membrane-associated small G protein that is indirectly coupled to receptor and nonreceptor tyrosine kinases.
• Ras activation is regulated by the levels of bound GTP and GDP.
• Several effectors of Ras have been identified, including Raf1, PI 3-kinase and RIN1.
• RIN1 (Ras interaction/interference) was identified as a Ras-interacting protein in yeast, and it has been shown to bind to the human H-Ras.
• This RIN1-Ras interaction is enhanced when Ras is bound to GTP.
• Unlike Raf1, RIN1 is localized primarily to the plasma membrane.
• RIN1 contains an SH2 domain and an amino-terminal region similar to consensus SH3 domains.
• RIN1 binds c-Abl and, like Raf1, interacts with 14-3-3 proteins.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.