FRAP is a large protein kinase that is the mammalian target of rapamycin, an immunosuppressant that blocks vessel restenosis and also has potential anticancer applications.
Rapamycin-insensitive companion of FRAP, also designated Rictor, shares homology with pianissimo from D.
discoidieum, STE20p from S.
pombe, and AVO3p from S.
cerevisiae.
Rictor forms a complex with FRAP, which is important in cell growth regulation as it integrates growth factor and nutrient derived signals.
The Rictor-FRAP complex plays a role in PKCalpha phosphorylation, directly phosphorylates Akt/PKB on Ser473 in vitro and facilitates Thr308 phosphorylation by PDK1.
It also may function as a drug target in tumors that have lost expression of PTEN, a tumor suppressor that opposes activation of Akt/PKB.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RICTOR. Species Crossreactivity: Human, mouse
