Upon activation, the small GTPase Rho D (also designated RhoHP1 and ARHD) contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution.
The effects of Rho D antagonize those of its family member, Rho A, by dissasembling actin stress fibers normally enhanced by Rho A.
Additionally, Rho D disengages focal adhesions, resulting in retardation of cell migration.
Accordingly, transfection of a constitutively active form of Rho D (designated Rho D G26V) reverses the invasive phenotype of Galphaolf induced cells, implying the possiblity of a therapeutic use for activated Rho D in counteracting tumor metastasis.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Rho D. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 156-201 of Human Rho D.
