Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors.
In mammals, G protein alpha, beta and y polypeptides are encoded by at least 16, 4 and 7 genes, respectively.
Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors.
Four Galpha GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (Galpha-interacting protein).
Each of these proteins has been shown to deactivate specific Galpha isoforms by increasing the rate at which they convert GTP to GDP.
RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Galpha i, Galpha o and Galpha t, but not Galpha s.
RGS10 increases the GTP hydrolytic activity of several members of the Galpha i subfamily including Galpha i-3, Galpha z, and Galpha o.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RGS4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human RGS4.
