84-2390-10　［取扱停止］RFFL (E3 Ubiquitin-Protein Ligase rififylin, Caspase Regulator CARP2, Caspases-8 and -10-Associated RING Finger Protein 2, CARP-2, FYVE-RING Finger Protein Sakura, Fring, RING Finger and FYVE-like Domain-Containing Protein 1, RING Finger Protein 189, RING Finger Protein 34-like, RFFL, RNF189, RNF34L) 100ul　225530

特徴

• Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction.
• The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s).
• RFFL, also known as RNF189, RNF34L or Rififylin, is a 363 amino acid protein that localizes to both the cytoplasm and to the membrane and contains one FYVE-type zinc finger, one RING-type zinc finger and two SAP domains.
• Expressed ubiquitously with increased expression in testis, spleen, ovary and prostate, RFFL functions as an E3 ubiquitin-protein ligase that interacts with caspase-8 and caspase-10 and targets them for proteasomal degradation.
• Multiple isoforms of RFFL exist due to alternative splicing events.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.