Renin is a highly specific endopeptidase that mediates the cleavage of the circulating substrate angiotensinogen to yield Angiotensin I.
Angiotensin-converting enzyme I (ACE) then completes the conversion from Angiotensin I to Angiotensin II which is significant in the regulation of electrolyte balance and blood pressure.
Sympathetic stimulation (beta1-adrenergic receptors), renal artery hypotension and decreases in sodium delivery to the distal tubules of the kidney signal the release of Renin.
The Renin-Angiotensin system (RAS) is essential for regulating blood volume, arterial pressure and normal cardiac and vascular function.
Renin is synthesized and secreted by modified smooth muscle cells in the juxtaglomerular apparatus (JGA) of the kidney.
Expression of Renin in other tissues, including brain, has been verified although the homeostatic role it may play is yet to be firmly established.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Renin. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 211-260 of Human Renin.
