The NFκB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein.
The DNAbinding subunit is functionally related to c-Rel p75 and Rel B p68.
The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105.
A second protein designated p52 (previously referred to as p49) has been identified that can act as an alternative NFκB subunit.
Rel B does not bind with high affinity to NFκB sites, but heterodimers between Rel B and p50 bind with an affinity comparable to that of p50 NFκB homodimers.
However, Rel B/p50 heterodimers, in contrast to NFκB heterodimers, transactivates transcription of promotors containing κB binding sites.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Rel B, phosphorylated S552. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human Rel B around the phosphorylation site of Serine 552.
