In humans, the RecQ helicase family includes WRN, BLM, RecQL1, RecQL4 and RecQL5 proteins, all of which contain a conserved helicase domain.
WRN and BLM have been demonstrated to be the responsible genes in Werner and Bloom syndromes, respectively.
RecQL1 and RecQL5 also belong to the human RecQ helicase family, but their correlation with genetic disorders, if any, is unknown.
The gene encoding human RecQL4, which maps to chromosome 8q24.3, is believed to be the gene responsible for the development of Rothmund-Thomson syndrome (RTS).
The levels of WRN, BLM, RecQL1, 4 and 5 are differentially up-regulated to guarantee genomic stability in cells that are transformed or actively proliferating.
In humans, RecQL1 and RecQL5 map to chromosome 12p12 and 17q25, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RECQL4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human RECQL4.
