Recoverin is implicated in the regulation of rhodopsin kinase activity that contributes to the adaptation to background illumination in retinal photoreceptor cells.
Recoverin, a Ca2+-binding photoreceptor protein, is recognized as an autoantigen of cancer-associated retinopathy (CAR), which is a rare paraneoplastic neurological syndrome characterized by the degeneration of retinal photoreceptors and associated with small-cell lung cancer.
Recoverin is a heterogeneously myristoylated protein that inhibits rhodopsin kinase by inhibiting its phosphorylation.
Ca2+ is required for Recoverin to bind rhodopsin kinase.
In addition, the binding of Recoverin-rhodopsin kinase is weakened by autophosphorylation of the kinase and is strengthened by the presence of ADP.
Upon accommodating two Ca2+ ions, Recoverin extrudes a myristoyl group and associates with the lipid bilayer membrane.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Recoverin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 120-160 of Human Recoverin.
