84-2389-60　［取扱停止］RBX1 (E3 Ubiquitin-Protein Ligase RBX1, FLJ60363, MGC13357, MGC1481, OTTHUMP00000028983, Protein ZYP, Rbx 1, Rbx1, RBX1_HUMAN, Regulator of Cullins 1, Ring box 1, Ring box 1 E3 Ubiquitin Protein Ligase, RING box Protein 1, RING Finger Protein 75, RING Finger Protein, RING-box Protein 1, Ringbox Protein 1, RNF 75, RNF75, ROC 1, ZYP Protein,) 50ul　225506

特徴

• RING-box protein 1 (RBX1 or ROC1) is an essential component of two distinct but structurally related E3 ubiquitin ligase complexes, the SCF complex and the CBC (VHL) complex (1).
• RBX1 mediates the neddylation of CUL1, which activates SCF E3 ligase by facilitating the ubiquitin transfer from E2 to substrates (2-4).
• The RING finger domain of RBX1 is required for ubiquitin ligation (5).
• Two evolutionarily conserved mammalian RBX family members, RBX1/ROC1 and RBX2/ROC2/SAG, have been identified (5).
• RBX1 is constitutively expressed and binds to CUL2/VHL, while stress-inducible RBX2 binds to CUL5/SOCS (6).
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.