Rb protein and p53 are both cell cycle checkpoint components.
Evidence suggests that p53 plays a role in regulating the phosphorylation of Rb by inducing p21 transcription, thus preventing Rb phosphorylation at the G1 to S transition.
Protein-protein interactions seem to be central in p53 cellular activities, as previously demonstrated with MDM2 and SV40 large T antigen.
Two novel proteins have been identified by their abilities to bind to p53 and/ or Rb.
Human RBQ-1 (also designated RBBP6) has been cloned as a novel protein that binds to the retinoblastoma (Rb) gene product.
A related mouse protein, P2P-R, also designated PACT (for p53 associated cellular protein-testis derived), has been shown to bind to both Rb and p53.
Recombinant P2P-R binds to wildtype p53 but not to mutant p53, and it can interfere with p53 specific DNA binding.
RBQ-1 may be a truncated human form of the P2P-R protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of RBBP6. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human RBBP6.
