Ras is a small GTP-binding protein involved in many cellular processes, including proliferation, differentiation and apoptosis.
Ras transmits signals of cell surface receptors by binding to a variety of effector molecules.
In addition to the well-characterized effectors Raf and PI-3 kinase, Ras also interacts with a group of homologous, noncatalytic proteins composed of RASSF1, RASSF2, RASSF3, AD037 and Nore1.
RASSF1 is a potential tumor suppressor gene that plays an important role in tumor pathogenesis.
Nore1 binds to Ras in response to EGF or serum stimulation, but its function has yet to be determined.
RASSF2 is a nuclear protein containing a Ras-associating domain and a SARAH domain.
RASSF2 isoform A is inactivated in colorectal cancer cells by CpG island promoter hypermethylation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RASSF2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 152-197 of Human RASSF2.
