Retinoids are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation.
Retinoic acid receptors (RARs) and retinoid X receptors (RXRs) are nuclear transcription factors that modulate the effects of retinoids (RA) on gene expression.
Most retinoid forms (including all trans RA, 9-cis RA, 4oxo RA and 3,4 dihydro RA) activate RAR family members, whereas RXR family members are activated by 9-cis-RA only.
RA binds RARs, inducing a change in receptor configuration that allows DNA binding and increased gene transcription from specific genes to occur.
RAR family members, which include RARalpha, RARbeta and RARγ, belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor.
Retinoid receptor expression is tissue specific; the skin expresses RARγ and RXRalpha.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of human RARbeta. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 321-370 of human RARβ.
