Lamellipodin, also called ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family.
The peripheral membrane protein mediates localized membrane signals and colocalizes at the tips of filopodia and lamellipodia with ENAH/VASP.
Aside from the main isoform, nine additional isoforms have been identified for Lamellipodin (RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc and RMO1-RAPH1).
RMO1-RAPH1 is expressed in a wide variety of tissues, most highly in brain, heart, ovary and developing embryo.
RMO1 is also widely expressed, with highest amounts in liver.
Lamellipodin is down regulated in breast and ovarian cancers and shows reduced expression in metastatic osteosarcomas in comparison to primary osteosarcoma tumors.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RAPH1. Species Crossreactivity: Human, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to residues in Human RAPH1.
