Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions including diabetes and other vascular disorders, as well as amyloidosis.
These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products).
RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells.
Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFκB.
Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 Ras and MAP kinase, which leads to activation of transcription.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human RAGE. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa254-308 of human RAGE
