84-2387-99　［取扱停止］RAD9A (Cell Cycle Checkpoint Control Protein, Cell Cycle Checkpoint Control Protein RAD9A, DNA Repair exonuclease rad9 Homolog A, hRAD 9, hRAD9, Rad 9, RAD 9A, RAD9 (S pombe) Homolog, RAD9 Homolog A, RAD9 Homolog, RAD9A, RAD9A) 100ul　225423

特徴

• DNA damage or incomplete replication of DNA results in the inhibition of cell cycle progression at the G1 to S or G2 to M phase checkpoints by conserved regulatory mechanisms.
• Chk1, Rad9 and Hus1 are involved in the signal transduction cascade that regulates cell cycle arrest at the G2 checkpoint.
• Chk1 functions as an essential component in the G2 phase DNA damage checkpoint, as it phosphorylates Cdc25C in response to DNA damage and thereby inhibits mitosis.
• Two related mammalian proteins, Hus1 and Rad9, share conserved sequence identity and function to the yeast homologs of the same names.
• In vivo, Rad9 is highly phosphorylated and directly associates with two other checkpoint control proteins, Rad1 and Hus1.
• Additionally, Rad9 associates with anti-apoptotic Bcl-2 family proteins Bcl-2 and Bcl-xL, but not with the pro-apoptotic Bax and Bad proteins.
• Overexpression of Rad9 induces apoptosis and indicates that Rad9 may have an additional role in regulating apoptosis after DNA damage.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.