Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation.
Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage.
BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51.
Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands.
Rad52 can interact with Rad51.
Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination.
Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA.
Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Rad51D. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 121-170 of Human Rad51D.
