The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair.
Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination.
In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3.
Rad51C binds single- and double-stranded DNA and preferentially binds 3'-end-tailed double-stranded DNA.
Rad51C is expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Rad51C. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 161-210 of Human Rad51C.
