84-2387-64　［取扱停止］Rad17 (Cell Cycle Checkpoint Protein RAD17, hRad17, RF-C/Activator 1 Homolog, RAD17, R24L) 50ul　225406

特徴

• DNA damage results in the arrest of cell cycle progression, allowing the damaged DNA to be repaired prior to replication.
• Checkpoints exist at several cell cycle phase transitions to maintain this genetic integrity.
• Rad9, Rad17, Rad24 and Mec3 are involved in activating the G1 and G2 checkpoints.
• Pol2 (also known as Dun2), encoding the catalytic subunit of DNA polymerase epsilon, plays a role in activating the S phase checkpoint.
• The protein kinase Rad53 (also designated Spk1, Mec2 or Sad1) is essential for both G2 and S phase arrest.
• Activation of Rad53 is regulated by Mec1 (also known as Esr1 and Sad3), a homolog of the human ATM protein.
• Pds1 and Mad2 both regulate checkpoints associated with incomplete spindle replication.
• Dun1, another protein kinase, plays a role in transducing the DNA damage signal.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.