PVRL1 is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily.
Human PVRL1 is identical to the poliovirus receptor-related protein (PRR) and is identified to be the alphaherpesvirus entry mediator.
PVRL1 constitutes a family consisting of at least PVRL1 1, 2 and 3.
PVRL1 2 and 3 are ubiquitously expressed, whereas PVRL1 1 is abundantly expressed in the brain.
PVRL1 1 exists as PVRL1 1alpha and 1beta/HIgR, produced by alternative splicing.
The cytoplasmic regions of PVRL1 1alpha, but not PVRL1 1beta/HIgR, have a C-terminal conserved motif (E/A-X-y-V).
This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton.
PVRL1 1, also designated HveC/ PRR1, allows the entry of herpes simplex virus type 1 (HSV-1) and HSV-2 into mammalian cells.
The interaction of virus envelope glycoprotein D (gD) with PVRL1 1 is an essential step in the process leading to membrane fusion; the gD binding site is located at the first Ig-like domain of PVRL1 1.
Both the transinteraction of PVRL1 and the interaction of PVRL1 with afadin are necessary for their co-localization with E-cadherin and catenins at adherens junctions.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PVRL1. Species Crossreactivity: Human, mouse, rat
