PUMA (Bcl-2 binding component 3, JFy1, PUMA/JFy1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway.
The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMAalpha, beta, y and delta.
Both PUMAalpha and PUMAbeta contain a BH3 domain, while PUMAy and PUMAdelta lack this domain.
The BH3 domain is essential for binding of PUMAalpha and PUMAbeta to Bcl-2 or Bcl-xL.
PUMA is an initiator of y-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo.
Bcl-2 family members generally regulate apoptosis and transmit death signals to mitochondria.
Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4).
The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PUMA. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 132-183 of Human PUMA.
