PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid mitochondrial protein.
During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm.
Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death.
It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis.
PTRH2 is a monomer that contains a N-terminal mitochondrial localization signal and a C-terminal UPF0099 domain.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PTRH2. Species Crossreactivity: Human
