Protein tyrosine phosphatases, or PTPs, are type I transmembrane proteins, membrane associated proteins or proteins localized in nuclei.
Examples of transmembrane PTPs are LAR, PTPalpha, PTPbeta, PTPγ, PTPδ, PTPε, PTPζ, PTPκ and PTPµ.
Transmembrane PTPs play diverse roles during development and in adult tissues.
Immunodepletion studies have suggested LAR to be a regulator of Insulin receptor phosphorylation.
PTPalpha activity is increased twofold in response to phorbol ester stimulation, resulting in serine phosphorylation either directly or indirectly by members of the PKC family.
Overexpression of v-H-ras and Neu, but not Myc or Int2, in mammary tumors has been shown to induce PTPε expression.
An alternative splicing event leads to a nervous tissue-specific chondroitin sulfate proteoglycan called phosphacan, which represents the amino terminal portion of PTPζ.
PTPκ and PTPµ share a conserved amino terminal 160 amino acid MAM domain which facilitates homophilic binding.
PTPµ localizes to points of cell contact and may be involved in regulating the assembly and disassembly of cadherin/catenin complexes in vivo.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PTPκ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human PTPκ.
