The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction.
This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases.
These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases.
In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein.
PTP-H1 shares homology with the cytoskeletal-associated proteins band 4.1, ezrin, and talin and has been shown to contain a PDZ and band 4.1 domain.
These domains are responsible for targeting proteins to the cytoskeleton-membrane interface, as well as mediating protein-protein interactions, recognizing C-terminal valine residues and binding to other PDZ domains.
Overexpression of PTP-H1 may reverse transformation induced by oncogenic protein-tyrosine kinases, such as the members of the src family.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PTPN3. Species Crossreactivity: Human, mouse
