The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction.
This is areversible process, involving both enzymes that phosphorylate proteins ontyrosine residues as well as a rapidly expanding family of protein tyrosinephosphatases.
These latter enzymes bear little resemblance to either theprotein serine and protein threonine phosphatases or to the acid and alkalinephosphatases.
In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein of ~40kDa molecular weight.
On the basis of sequence analysis, PTP1B expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, ahomolog of the neural adhesion molecule (NCAM).
PTPase 1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PTP1B. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 20-70 of Human PTP1B.
