Prothymosin a (PTalpha) is a nuclear protein that is widely expressed in mammalian tissues, including kidney, liver, spleen, normal lymphocytes, human T-cell leukemia virus-infected T cells and myeloma cells.
The human PTalpha gene maps to chromosome 2 and encodes a protein that exhibits punctuated nuclear distribution, which correlates to transcription sites.
PTalpha is a chromatin-remodeling protein that was initially thought to mediate T-lymphocyte maturation, but subsequently, has been shown to be involved in cell cycle progression, proliferation and cell differentiation.
PTalpha is thought to be transported into the nucleus by the karyopherin beta1-Rch-1 complex, where it associates with histones H2A, H2B, H3 and H4.
Also, PTalpha is phosphorylated on Thr 7 and Thr 12 or 13 by Prothymosin alpha-phosphorylating kinase (PTalphaK) in a mitogen-activating pathway.
The amino terminus of PTalpha is cleaved to produce a secreted, biologically active peptide thymosin alpha1, which may be used as an immunomodulator in cancer patients and patients with chronic active hepatitis, or as an immunoenhancer of vaccines in immunocompromised individuals.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PTMA. Species Crossreactivity: Human, mouse, rat
