84-2386-08　［取扱停止］PTBP1 (57kD RNA Binding Protein PPTB 1, 57kD RNA Binding Protein PPTB1, 57kD RNA-Binding Protein PPTB-1, Heterogeneous Nuclear Ribonucleoprotein I, Heterogeneous Nuclear Ribonucleoprotein Polypeptide I, hnRNP I, HNRNP-I, HNRNPI, HNRPI, MGC10830, MGC8461, Polypyrimidine tract Binding Protein (heterogeneous Nuclear Ribonucleoprotein I), Polypyrimidine Tract Binding ...　225324

特徴

• This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
• The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA).
• These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
• While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm.
• The hnRNP proteins have distinct nucleic acid binding properties.
• The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs.
• This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway.
• It may also promote the binding of U2 snRNP to pre-mRNAs.
• This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure.
• Alternatively spliced transcript variants encoding different isoforms have been described.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.