BRIDGE-1, a protein homologous to a previously cloned proteasome subunit p27 is important in regulating Insulin and other islet genes in the pancreas.
BRIDGE-1 is highly expressed in pancreatic b-cells and is predominantly located in the nucleus, although lower levels are expressed in the cytoplasm.
BRIDGE-1 contains a conserved PDZ-like domain that mediates protein-protein interactions in a variety of intracellular signaling processes, including the transactivational activity of E2A.
One mechanism of the activation of gene transcription in pancreatic b-cells is the interaction of E2A with coactivating proteins such as CBP, p300 and BRIDGE-1.
The interaction of E12 and E47, members of the E2A family of transcription factors, with the PDZ-domain of BRIDGE-1 suggest a novel mechanism for Insulin gene regulation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PSMD9. Species Crossreactivity: Human, mouse, rat
