The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation.
It consists of four stacked rings, each with seven distinct subunits.
The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits.
While the catalytic sites are located on the beta rings, the alpha subunits are important for assembly and as binding sites for regulatory proteins.
Seven different alpha and ten different beta proteasome genes have been identified in mammals.
PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome.
PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins ).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PSMA3. Species Crossreactivity: Human, mouse, rat
