84-2385-16　［取扱停止］PSAP (A1 Activator, Cerebroside Sulfate Activator, Co-beta-glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase Activator, ProActivator Polypeptide, ProActivator Polypeptide Precursor, Prosaposin (sphingolipid Activator Protein 1), prosaposin (Variant Gaucher Disease and Variant metachromatic leukodystrophy), Prosaposin, Protein A, Protein C, PSAP, ...　225278

特徴

• The saposin family includes four structurally related activator proteins, saposin A, B, C and D, that are cleaved from the single precursor protein prosaposin.
• The gene encoding human prosaposin maps to chromosome 10.
• Prosaposin is synthesized as a protein that is posttranslationally modified to a shorter form and then further glycosylated to yield a secretory product.
• This form subsequently undergoes partial proteolysis to produce saposin A, B, C and D.
• Each saposin family member acts in conjunction with hydrolase enzymes to facilitate the brea own of glycosphingolipids within the lysosome.
• The saposins modify the environment of target lipids to make them accessible to the active sites of specific enzymes.
• Saposin A and C are involved in the hydrolysis of glucosylceramidase, and defects in saposin C are linked to Gaucher’s disease.
• Saposin B facilitates the hydrolysis of the sulfate group from cerebroside sulfate, and defects in this protein are responsible for a form of metachromatic leukodystropy, a progressive neurodegenerative condition.
• Saposin D may stimulate the hydrolysis of sphingomyelin and ceramide, but its exact physiological role is not clear.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (Paraffin): 1:50-1:200Immunofluorescence: MCF cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.