84-2384-97　［取扱停止］PRPF31 (hPrp 31, hPrp31, NY BR 99, Pre mRNA processing Factor 31, Pre mRNA processing Factor 31 Homolog (yeast), Pre mRNA processing Factor 31 Homolog, Pre-mRNA-processing Factor 31, Precursor mRNA-processing Factor 31, S. cerevisiae, Homolog of, Protein 61K, PRP 31, PRP31, PRP31 pre mRNA processing Factor 31 Homolog (yeast), PRP31 pre mRNA processing Factor 31 Ho ...　225269

特徴

• PRPF31 (PRP31 pre-mRNA processing factor 31 homolog), also known as RP11, PRP31 or NY-BR-99, is a ubiquitously expressed protein that localizes to the nucleus and is found in Cajal bodies and speckles.
• PRPF31 is involved in pre-mRNA splicing and functions as a component of the U4/U6.U5 tri-snRNP (small nuclear ribonucleoprotein) complex.
• More specifically, PRPF31 is believed to mediate the tethering of the tri-snRNP to the spliceosome (a large ribonucleoprotien responsible for catalyzing the splicing of pre-mRNA), thereby assisting in the assembly of the mature spliceosome.
• Mutations in the gene encoding PRPF31 result in autosomal dominant retinitis pigmentosa type 11 (RP11), which leads to photoreceptor cell degeneration.
• RP11 patients initially exhibit a loss of their midperipheral visual field as well as night vision blindness.
• The disease eventually progresses to the loss of far peripheral visual field and finally the loss of central vision.
• This suggests that PRPF31 is a key player in the pre-mRNA splicing of photoreceptor-specific genes.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.