The prokineticin receptors, PKR1 (GPR73a) and PKR2 (GPR73b), are G protein-coupled receptors responsible for mediating the signal transduction of both EG-VEGF and Prokineticin-2.
PKR1 and PKR2 share 87% sequence identity.
PKR1 is predominantly expressed in the peripheral tissues and PKR2 is typically expressed in the CNS.
Both receptors are found in the testis.
Upon ligand binding, PKR1 and PKR2 associate with G protein and can promote intracellular calcium mobilization, stimulate phosphoinositide turnover and activate the MAPK pathway.
These receptors play a role in a variety of physiological events such as intestinal contraction, ingestive behavior, spermatogenesis, angiogenesis, circadian rhythm, neuronal survival and hyperalgesia.
PKR1 may promote cardiomyocyte survival.
PKR2 is essential for the normal development of the olfactory bulb.
Mutations in the gene encoding PKR2 may result in Kallmann syndrome type 3.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PROKR2. Species Crossreactivity: Human, mouse, rat
