Profilins (Profilin-1, Profilin-2 and Profilin-3) act as nucleotide exchange factors that use ATP to charge Actin, a process that regulates Actin polymerization by subsequently sequestering the Actin monomer.
Profilin-2, also known as PFN2 or PFL, is a 140 amino acid protein that is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle.
Like other members of the Profilin family, Profilin-2 functions as an Actin monomer-binding protein that influences the structure of the cytoskeleton by regulating Actin polymerization in response to extracellular signals.
High levels of Profilin-2 inhibit Actin polymerization, while lower levels enhance Actin polymerization, suggesting that the expression of Profilin-2 is regulated by the need for polymerization within the cell.
Two isoforms of Profilin-2 exist due to alternative splicing events.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Profilin-2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human Profilin-2.
