Thrombomodulin (TM) is an endothelial specific receptor that forms a complex with Thrombin, a protein with procoagulant, inflammatory and anticoagu-lant effects.
The TM/Thrombin complex activates Protein C (PC) to generate activated Protein C (APC) and initiate the APC anticoagulant pathway.
APC attenuates Thrombin formation through the inactivation, by limited proteolysis, of two significant cofactors of blood clot formation, Factor Va and Factor VIIIa.
This process is augmented by the activity of the endothelial cell Protein C receptor (EPCR), which binds both PC and APC with high affinity.
The EPCR gene maps to human chromosome 20q11.2 and encodes an anticoagulant that is preferentially expressed on large blood vessel endothelium in the heart and lung with some expression in capillaries in the lung and skin.
EPCR, also designated CCD41 in mouse, is a member of the major histocompatibility complex and displays significant homology to CD1.
Soluble plasma EPCR is thought to inhibit the membrane-bound EPCR activation of the APC pathway.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PROCR. Species Crossreactivity: Human
