The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases.
PRKy (protein kinase, y-linked) and PRKX (protein kinase, X-linked) are members of the Ser/Thr protein kinase family, both of which belong to the subfamily of cAMP-dependent kinases.
Encoded by a gene that is located near the pseudoautosomal region on chromosome y, PRKy contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins.
Functioning in a similar manner to PRXy, PRKX contains one protein kinase domain and is essential for macrophage differentiation and renal epithelial cell migration.
Defects in the genes encoding PRKy and PRXX are associated with sex reversal disorder, namely XX in males and Xy in females.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PRKX. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 308-353 of Human PRKX.
