The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes.
The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate.
In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression.
PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization.
The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system.
PRX II is expressed in testis, while PRX III shows expression in lung.
PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression.
Upregulated protein levels of PRX I and II in Alzheimer’s disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.
The human PRX IV gene is abundantly expressed in many tissues.
PRX IV exists as a precursor protein, which is only detected in testis, and a processed secreted form.
PRX V also exists as two forms, designated long and short.
Like PRX IV, the long form of PRX V is highly expressed in testis.
The short form of PRX V is more widely expressed, with high expression in liver, kidney, heart and lung.
PRX VI, a 1-Cys peroxiredoxin (also known as antioxidant protein 2 or AOP2), is highly expressed in most tissues, particularly in epithelial cells.
Localized to the cell cytosol, PRX VI functions independently of other peroxiredoxins and antioxidant proteins, specializing in antioxidant defense, lung phospholipid metabolism and protection of keratinocytes from cell death induced by reactive oxygen species.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PRDX5. Species Crossreactivity: Human
