The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its role in signal transduction pathways and high frequency of mutations in human malignancies.
The founding members of the Ras family (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins.
Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit ~50% amino acid homology to Ras.
The mammalian Rho proteins (Rho A, B, C, 6, 7 and 8) are ~30% homologous to Ras and are expressed in a wide range of cell types.
Three Rho-related GTPases Rnd1 (Rho 6), Rnd2 (Rho 7), and Rnd3 (Rho 8 or Rho E) form a distinct branch of the Rho family since they differ from other Rho proteins in size, charge, and biochemical properties.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Pragmin, phosphorylated Y413. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from human Pragmin around the phosphorylation site of Tyrosine 413.
