The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions.
The protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit, and a catalytic subunit.
PR48 is a 48kDa regulatory subunit of protein phosphatase 2A (PP2A).
PP2A activity is required for the initiation of DNA replication in yeast, viral, and vertebrate systems.
PR48 localizes to the nucleus and binds specifically to Cdc6, a highly conserved protein which is required for the formation of prereplicative complexes.
PR48 is considered to be involved in the dephosphorylation of Cdc6 by PP2A, a process important to the control of DNA replication in mammalian cell.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PR48. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 370-412 of Human PR48.
