In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the protein phosphatases.
In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit.
Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C.
An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
The PP2A family comprises subfamily members PP2Aalpha and PP2Abeta.
The PP2A catalytic subunit associates with a variety of regulatory subunits.
Regulatory subunits include PP2A-A-alpha and -A-beta, PP2A-B-alpha and -B-beta, PP2A-C-alpha and -C-beta, PP2A-B56-alpha, -B56-beta, -B56-y and -B56-delta.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human PPP2R5A. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa363-408 of human PPP2R5A.
