Protein phosphatase 1 (PP1) is a serine-threonine protein phosphatase that plays a central role in mediating the effects of insulin on glucose and lipid metabolism.
PTG (protein targeting to glycogen) was cloned from 3T3-L1 adipocytes as a protein that binds to the PP1 catalytic subunit.
The human homolog of PTG, designated PPP1R5, has been shown to bind to PP1 and to modulate its specificity.
PTEN/PPP1R5 shows 42% identity to the glycogen binding subunit, G(L), of rat liver PP1.
PTG is expressed predominantly in insulin-sensitive tissues, and it localizes PP1 to glycogen.
PTG also has been shown to interact with several enzymes involved in the hormonal regulation of glycogen metabolism, including phosphorylase kinase, phosphorylase A and glycogen synthase.
These data indicate a role for PTG in glycogen metabolism, possibly that of a molecular scaffold.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PPP1R3C. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 40-93 of Human PPP1R3C.
