Protein phosphatase inhibitor-1 (also designated inhibitor of protein phospha-tase 1, IPP-1 and I-1) plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase at Threonine 35.
In addition, the proline-directed kinases Cdk1, Cdk5, and mitogen-activated protein kinase (MAPK) mediate in vitro phosphorylation of IPP-1 at the phylogenetically conserved position Serine 67.
In striatal tissues, glutamate-dependent regulation of N-methyl-d-aspartic acid-type channels influences IPP-1 phosphorylation at Ser 67.
The localization and expression of IPP-1 suggests that it may play discrete roles in certain regions and developing stages of the brain, independent of the regulation of protein phosphatase type 1 (PP-1).
PP-1 binds to both phosphorylated and dephosphorylated IPP-1.
Conversion of PP-1 to a Mn++-dependent state appears to play a role in its regulation by IPP-1.
IPP-1 attenuates the activity of glycogen phosphorylase and is thought to be important in the hormonal control of glycogen metabolism.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PPP1R1A. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human PPP1R1A.
