In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the protein phosphatases.
In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit.
Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C.
An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
The PP1 family is comprised of subfamily members PP1alpha, PP1beta and PP1γ, which are MgATP-dependent enzymes.
PP1 inactivity is maintained through its association with the inhibitory protein NIPP-1 (nuclear inhibitor of PP1).
Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PPP1CA. Species Crossreactivity: Human, mouse, rat
