84-2383-75　［取扱停止］PPM2C ([Pyruvate Dehydrogenase [acetyl-transferring]]-Phosphatase 1, Mitochondrial, PDP 1, Protein Phosphatase 2C, Pyruvate Dehydrogenase Phosphatase Catalytic Subunit 1, PDPC 1, PDP1, PDP, PPM2C) 100ul　225201

特徴

• Pyruvate dehydrogenase phosphatase (PDP) is a serine phosphatase that catalyzes the dephosphorylation and reactivation of the alpha subunit of the E1 component of the mitochondrial pyruvate dehydrogenase multienzyme complex.
• PDP is a heterodimer that consists of catalytic and regulatory subunits.
• PDPc (pyruvate dehydrogenase phosphatase, catalytic subunit 1), also known as Protein phosphatase 2C, is a 537 amino acid protein that is localized within the mitochondrial matrix.
• PDPc is stimulated by calcium binding and utilizes two magnesium ions as cofactors.
• PDPc efficiently dephosphorylates all three phosphorylation sites located on the alpha chain of the E1 component, which simultaneously activates pyruvate dehydrogenase to convert pyruvate to acetyl-CoA for utilization in the Kreb’s Cycle.
• Defects in the gene encoding PDPc are the cause of pyruvate dehydrogenase phosphatase deficiency, which results in lactic acidosis and neurological dysfunction.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.