PPAR gamma is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer.
PPAR gamma activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues.
A key component in adipocyte differentiation and fat-specific gene expression, PPAR gamma may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.
A Pro12Ala polymorphism of the PPAR gamma2 gene has been reported to reduce transactivation activity in vitro.
This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes.
In addition, the Pro12Ala variant of the PPAR gamma2 gene maybe correlated with abdominal obesity in type 2 diabetes.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PPARγ, phosphorylated S112. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human PPARγ around the phosphorylation site of Serine 112.
