PPA1 (Pyrophosphate phospho-hydrolase 1), also known as IOPPP (inorganic pyrophosphatase), PP1, PP or PPase, belongs to the PPase family of inorganic pyrophosphatases.
Inorganic pyrophosphatases catalyze the intracellular conversion of pyrophosphate to inorganic phosphate, a key reaction for phosphate metabolism in cells.
PPA1 is a ubiquitously expressed protein that localizes to the cytoplasm and is required for cell growth.
It exists as a homodimer exhibiting magnesium dependent activity.
The binding of two magnesium ions is required to stimulate PPA1 activity; however, both subunits in the homodimer are capable of binding four magnesium ions.
The additional ions are useful in forming complexes with substrates and products.
In addition, the activity of PPA1 can be inhibited by calcium.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PPA1. Species Crossreactivity: Human, mouse, rat
