Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III.
Each polymerase is responsible for the synthesis of a different class of RNA.
RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes.
POLR3K, also known as DNA-directed RNA polymerase III subunit RPC11, is a 108 amino acid nuclear protein that is one of the seventeen subunits that comprise Pol III.
Specifically, POLR3K is a DNA-dependent RNA polymerase that uses the four ribonucleoside triphosphates to catalyze the transcription of DNA to RNA.
The gene encoding POLR3K maps to human chromosome 16, which encodes over 900 genes in ~90 million base pairs and is associated with a variety of genetic disorders.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of POLR3K. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human POLR3K.
