RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes.
Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors.
Pol II can coordinate 10 to 14 subunits.
This complex interacts with the promoter regions of genes and a variety of elements and transcription factors.
POLR2I (polymerase (RNA) II (DNA directed) polypeptide I), also known as RPB9 or hRPB14.5, is a 125 amino acid nuclear protein belonging to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Component of RNA polymerase II, POLR2I catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
POLR2I is part of the upper jaw surrounding the central large cleft and is thought to grab the incoming DNA template.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of POLR2I. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human POLR2I.
